Utility of phenotypic methods in detection of metallo-beta-lactamases in gram-negative bacteria

phenotypic and genotypic detection of metallo-beta-lactamases among imipenem-resistant gram negative isolates

background :   imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams....

phenotypic and genotypic detection of metallo-beta-lactamases among imipenem resistant gram negative isolates

background :   imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams....

Phenotypic and Genotypic Detection of Metallo-Beta-Lactamases in Carbapenem Resistant Acinetobacter baumannii

The Importance of Extended-Spectrum β-lactamases in Gram-Negative Enteric Bacilli and the Phenotypic Methods of detection

The production of β-lactamase enzymes is the main mechanism of resistance to β-lactam antibiotics in gramnegative bacilli. Therefore, it is important to identify the β-lactamases-producing bacteria for the treatment of caused infections. This article aimed to review the recent literature and guidelines regarding phenotypic detection of β-lactamases in common gram-negative bacilli in clinical sa...

Phenotypic detection of carbapenem-susceptible metallo-beta-lactamase-producing gram-negative bacilli in the clinical laboratory.

Rapid detection of metallo-beta-lactamase (MBL)-producing gram-negative pathogens is critical to prevent their widespread dissemination. Thus far, no standardized phenotypic method is available, and previously reported techniques have poor sensitivity for detecting carbapenem-susceptible MBL-carrying isolates, an increasingly described phenomenon. We developed a phenotypic detection method usin...